Abbreviation: cGMP
Target Species: Universal
Usage: This ELISA kit applies to the in vitro quantitative determination of cGMP concentrations in serum, plasma and other biological fluids.
Tested Sample Types: serum, plasma and other biological fluids
Assay Time: 2.5h
Sensitivity: 0.47 pmol/mL
Detection Range: 0.78-50 pmol/mL
Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal cGMP. During the reaction, Universal cGMP in the sample or standard competes with a fixed amount of Universal cGMP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal cGMP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal cGMP in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Cross reactivity: This kit recognizes Universal cGMP in samples.No significant cross-reactivity or interference between Universal cGMP and analogues was observed
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