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Applications: Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.
Background: CDC7 (cell division cycle 7-related protein kinase), also known as DDK (Dbf4/Drf1-dependent kinase), is a serine/threonine kinase involved in cell cycling. Once activated by binding to DbF4b it phosphorylates MCM2 (minichromosome maintenance 2), a protein of the MCM helicase complex, and DNA synthesis is initiated. It is also crucial for the activation of ATR (ataxia telangiectasia and RAD3 related protein) and CHK1 (checkpoint kinase 1). It is involved at the S-phase checkpoint, allowing for controlled and faithful DNA replication. It is found at high levels in cancer cell lines and tumors, where it can contribute to cell cycle deregulation and genome instability. Knockdown of this protein in cancer cell lines leads to cell death, via an ATR-dependent mechanism. Studies in Ewing sarcoma, the second most common bone cancer in children and teenagers, showed that inhibition of CDC7 leads to mitotic catastrophe and increased DNA damage. The development of inhibitors targeting CDC7 is an ongoing area of research, with the goal of improving specificity and reducing off-target effects.
Contraindications: The final concentration of DMSO in the assay should not exceed 1%.
Description: The Chemi-Verse™ CDC7 Kinase Assay Kit is designed to measure CDC7 (cell division cycle 7-related protein kinase) kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough recombinant purified CDC7, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Uniprot: O00311
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
References: Martin J., et al., 2022 Cell Death Discovery 8:85.
Cheng A., et al., 2018 EBioMedicine 36:241-251.
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