Products from BPS Bioscience require a minimum order value above 400€
Applications: Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.
Background: FYN, also known as p59-FYN, SLK, SYN, is a member of the SFK (Src family of kinase) non-receptor tyrosine kinase protein family. It is involved in cell growth, adhesion and cytoskeletal remodeling, axon guidance and synaptic function, platelet activation and T-cell receptor signaling. Its levels are regulated by miRNA, transcription factors such as STAT5 (signal transducer and activator of transcription 5) and RUNX2 (runt-related transcription factor 2), and the Cbl (Casitas B-lineage lymphoma) ubiquitin E3 ligase. It is normally a tumor suppressor, but dysfunction can lead to prostate cancer, leukemia, pancreatic cancer, breast cancer and others. Downregulation of miR-153-3p levels leads to increased levels of FYN in ESCC (esophageal squamous cell carcinoma). On the other hand, miR-381 downregulates FYN and can make breast cancer cells more susceptible to doxorubicin. FYN activity is regulated by multiple upstream proteins, including CD16 and KRAS (Kirsten rat sarcoma), and in turn can regulate many substrates that are involved in cell growth. This protein is also involved in the development of drug resistance, as in the resistance to imatinib in leukemia. Several inhibitors have been developed to target FYN, with saracatinib being in phase II clinical trials for prostate cancer. Inhibition of FYN is of interest in Alzheimer's disease (AD), as this protein acts downstream on the pathological pathway of amyloid beta (Aβ) that leads to the disease. Oligomeric Aβ binds to PrPC (cellular prion protein) and activates a pathway dependent on mGluR5 (metabotropic glutamate receptor 5) and FYN. Thus, targeting FYN with saracatinib for the treatment of AD is in clinical trial. These multiple crucial roles make this protein an attractive therapeutic target in cancer and AD.
Contraindications: The final concentration of DMSO in the assay should not exceed 1%.
Description: The Chemi-Verse™ FYN Kinase Assay Kit is designed to measure FYN (FYN proto-oncogene, Src family tyrosine kinase) tyrosine kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified FYN, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Uniprot: P06241
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
References: Nygaard H., 2018 Biol Psychiatry 83(4): 369-376.
Feng S. and Fu Y., 2023 Journal of Translational Medicine 21:84.
English
Deutsch
