Result Type: Quantitative
Detection Instrument: Fluorescence Microplate reader
Tested Sample Types: serum, plasma and animal tissue
Assay Time: 30 min
Sensitivity: 0.06 umol/L
Detection Range: 0.06-10 umol/L
Test Principle: Choline and acetylcholine play important roles in many biological processes. Choline is an essential nutrient, often grouped with B complex vitamins, that plays a key role in many biological processes. Choline is a precursor to the synthesis of acetylcholine (AchE), an important neurotransmitter in the peripheral and central nervous systems.Choline is measured by enzyme coupling reaction to generate fluorescent products in this kit, and the content of fluorescent substances is proportional to the presence of choline. Acetylcholinesterase was added to the reaction to hydrolyze acetylcholine into choline and acetic acid, and the acetylcholine content was further calculated by measuring the total amount of choline and acetylcholine.
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