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Applications: This kit is useful for screening for inhibitors of B7-1 binding to CTLA4.
Description: The activation of naíve T cells requires two signals, the specific T cell receptor recognition of MHC/Antigen on the surface of the antigen-presenting cell (APC), and the binding of B7-1 (CD80) ligand on the APC with the CD28 receptor on the T cell surface. Conversely, binding of CTLA4 to B7-1 on the T-cell surface results in an inhibitory signal and prevents T-cell activation. CTLA4B7-1 interaction is an important drug target for the regulation of the host's response to cancer. The CTLA4 [Biotinylated]:B7-1 Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of CTLA4:B7-1 signaling. The key to this kit is the high sensitivity of detection of biotin-labeled CTLA4 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, B7-1 is coated on a 96-well plate. Next, CTLA4[B] is incubated with B7-1 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.
Storage Stability: Stable at least 6 months from date of receipt when stored as directed.
Supplied As: This kit comes in a convenient 96-well format, with biotin-labeled CTLA4 (CTLA4[B]), purified B7-1, streptavidin-labeled HRP, and assay buffer for 100 binding reactions.
Biosafety Level: Not applicable (BSL-1)
References: 1. Linsley, P.S., et al. J. Exp. Med. 1991, 174(3): 561-569.
2. Hurwitz, A.A., et al. Canc. Res. 2000, 60: 2444-2448.
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