HS Mouse TNF-alpha(Tumor Necrosis Factor Alpha) ELISA Kit

HS Mouse TNF-alpha(Tumor Necrosis Factor Alpha) ELISA Kit
SKU
ELSE-HSEL-M0009-96
Packaging Unit
96 T
Manufacturer
Elabscience Biotechnology

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Abbreviation: TNF-α

Target Synonym: DIF; TNF-alpha; TNFA;TNFSF2;TNF;TNFa

Target Species: Mouse

Usage: This ELISA kit applies to the in vitro quantitative determination of Mouse TNF-α concentrations in serum, plasma and other biological fluids.

Tested Sample Types: serum, plasma and other biological fluids

Assay Time: 3.5h

Sensitivity: 2.76 pg/mL

Detection Range: 4.69-300 pg/mL

UNIProt ID: P06804

Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TNF-α. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TNF-α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TNF-α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse TNF-α. You can calculate the concentration of Mouse TNF-α in the samples by comparing the OD of the samples to the standard curve.

Cross reactivity: This kit recognizes Mouse TNF-α in samples. No significant cross-reactivity or interference between Mouse TNF-α and analogues was observed.
More Information
SKU ELSE-HSEL-M0009-96
Manufacturer Elabscience Biotechnology
Manufacturer SKU ELA-E-HSEL-M0009-96
Package Unit 96 T
Quantity Unit STK
Application ELISA
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