Human ACTN3 (Actinin Alpha 3) CLIA Kit

Abbreviation: ACTN3

Target Synonym: alpha-actinin skeletal muscle isoform 3;F-actin cross-linking protein;MGC117002;MGC117005;

Target Species: Human

Usage: This ELISA kit applies to the in vitro quantitative determination of Human ACTN3 concentrations in Tissue homogenates.

Tested Sample Types: Tissue homogenates

Assay Time: 3.5h

Sensitivity: 46.88 pg/mL

Detection Range: 78.13-5000 pg/mL

UNIProt ID: Q08043

Test Principle: This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ACTN3. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ACTN3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ACTN3, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human ACTN3. You can calculate the concentration of Human ACTN3 in the samples by comparing the RLU value of the samples to the standard curve.

Cross reactivity: This kit recognizes Human ACTN3 in samples.No significant cross-reactivity or interference between Human ACTN3 and analogues was observed

• SKU: ELSE-CL-H0215-48
• Manufacturer: Elabscience Biotechnology
• Manufacturer SKU: ELA-E-CL-H0215-48
• Green Labware: No
• Package Unit: 48 T
• Quantity Unit: PAK
• Application: Chemiluminescence Immunoassays (CLIA)