Human alphaFP(Alpha-Fetoprotein) ELISA Kit

Abbreviation: αFP

Target Synonym: AFP;Alpha-Fetoprotein;

Target Species: Human

Usage: This ELISA kit applies to the in vitro quantitative determination of Human αFP concentrations in serum, plasma.

Tested Sample Types: serum, plasma

Assay Time: 1.5h

Sensitivity: 0.47 ng/mL

Detection Range: 0.78-50 ng/mL

UNIProt ID: P02771

Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human αFP. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human αFP are added to the micro ELISA plate wells. Human αFP in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human αFP.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Human αFP. The concentration of Human αFP in the samples is then determined by comparing the OD of the samples to the standard curve.

Cross reactivity: This kit recognizes Human αFP in samples.No significant cross-reactivity or interference between Human αFP and analogues was observed

• SKU: ELSE-OSEL-H0015-48
• Manufacturer: Elabscience Biotechnology
• Manufacturer SKU: ELA-E-OSEL-H0015-48
• Green Labware: No
• Package Unit: 48 T
• Quantity Unit: PAK
• Application: ELISA