Abbreviation: C1q
Target Species: Human
Usage: This ELISA kit applies to the in vitro quantitative determination of Human C1q concentrations in Serum, plasma and other biological fluids.
Tested Sample Types: Serum, plasma and other biological fluids
Assay Time: 3.5h
Sensitivity: 1.88 ng/mL
Detection Range: 3.13-200 ng/mL
UNIProt ID: P02745,P02746,P02747
Test Principle: This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human C1q. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human C1q and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human C1q, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human C1q. You can calculate the concentration of Human C1q in the samples by comparing the RLU value of the samples to the standard curve.
Cross reactivity: This kit recognizes Human C1q in samples.No significant cross-reactivity or interference between Human C1q and analogues was observed
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