Human C4b (Complement Component 4b) CLIA Kit

Abbreviation: C4b

Target Synonym: C4-B;CPAMD3;C4(Chido Blood Group);Basic C4;

Target Species: Human

Usage: This ELISA kit applies to the in vitro quantitative determination of Human C4b concentrations in serum, plasma and other biological fluids.

Tested Sample Types: serum, plasma and other biological fluids

Assay Time: 3.5h

Sensitivity: 0.56 ng/mL

Detection Range: 0.94-60 ng/mL

UNIProt ID: P0C0L5

Test Principle: This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human C4b. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human C4b and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human C4b, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human C4b. You can calculate the concentration of Human C4b in the samples by comparing the RLU value of the samples to the standard curve.

Cross reactivity: This kit recognizes Human C4b in samples.No significant cross-reactivity or interference between Human C4b and analogues was observed

• SKU: ELSE-CL-H0575-48
• Manufacturer: Elabscience Biotechnology
• Manufacturer SKU: ELA-E-CL-H0575-48
• Green Labware: No
• Package Unit: 48 T
• Quantity Unit: PAK
• Application: Chemiluminescence Immunoassays (CLIA)