Abbreviation: CD8
Target Species: Human
Usage: This ELISA kit applies to the in vitro quantitative determination of Human CD8 concentrations in serum, plasma and other biological fluids.
Tested Sample Types: serum, plasma and other biological fluids
Assay Time: 3.5h
Sensitivity: 0.75 ng/mL
Detection Range: 1.25-80 ng/mL
UNIProt ID: P01732,P10966
Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CD8. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CD8 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CD8, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CD8. You can calculate the concentration of Human CD8 in the samples by comparing the OD of the samples to the standard curve.
Cross reactivity: This kit recognizes Human CD8 in samples. No significant cross-reactivity or interference between Human CD8 and analogues was observed
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