Abbreviation: Cortisol
Target Synonym: 11β;17α;21-Trihydroxypregn-4-ene-3;20-dione;
Target Species: Human
Usage: This ELISA kit applies to the in vitro quantitative determination of Human Cortisol concentrations in serum, plasma, saliva, urine.
Tested Sample Types: serum, plasma, saliva, urine
Assay Time: 1.5h
Sensitivity: 2.87 ng/mL
Detection Range: 6.25-400 ng/mL
Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human Cortisol. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human Cortisol are added to the micro ELISA plate wells. Human Cortisol in samples (or standards) competes with a fixed amount of Cortisol on the solid phase supporter for sites on the HRP linked detection antibody specific to Cortisol. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Human Cortisol in the samples is then determined by comparing the OD of the samples to the standard curve.
Cross reactivity: This kit recognizes Human Cortisol in samples.No significant cross-reactivity or interference between Human Cortisol and analogues was observed
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