Abbreviation: E2
Target Synonym: Oestradiol;E2;17β-Estradiol;Estra-1;3;5(10)-triene-3;17β-diol;
Target Species: Human
Usage: This ELISA kit applies to the in vitro quantitative determination of Human E2 concentrations in serum, plasma, saliva, urine.
Tested Sample Types: serum, plasma, saliva, urine
Assay Time: 1.5h
Sensitivity: 13.22 pg/mL
Detection Range: 31.25-2000 pg/mL
Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human E2. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human E2 are added to the micro ELISA plate wells. Human E2 in samples (or standards) competes with a fixed amount of E2 on the solid phase supporter for sites on the HRP linked detection antibody specific to E2. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Human E2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Cross reactivity: This kit recognizes Human E2 in samples.No significant cross-reactivity or interference between Human E2 and analogues was observed
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