Abbreviation: F13
Target Synonym: Factor XIII;fibrin stabilizing factor;F13;Laki-Lorand factor;
Target Species: Human
Usage: This ELISA kit applies to the in vitro quantitative determination of Human F13 concentrations in serum, plasma and other biological fluids.
Tested Sample Types: serum, plasma and other biological fluids
Assay Time: 3.5h
Sensitivity: 15 pg/mL
Detection Range: 25-1600 pg/mL
UNIProt ID: P00488,P05160
Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human F13. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human F13 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human F13, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human F13. You can calculate the concentration of Human F13 in the samples by comparing the OD of the samples to the standard curve.
Cross reactivity: This kit recognizes Human F13 in samples. No significant cross-reactivity or interference between Human F13 and analogues was observed
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