Human IL-8(Interleukin 8) ELISA Kit

Abbreviation: IL-8

Target Synonym: IL8/CXCL8

Target Species: Human

Usage: This ELISA kit applies to the in vitro quantitative determination of Human IL-8 concentrations in serum, plasma.

Tested Sample Types: serum, plasma

Assay Time: 1.5h

Sensitivity: 9.38 pg/mL

Detection Range: 15.63-1000 pg/mL

UNIProt ID: P10145

Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-8. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human IL-8 are added to the micro ELISA plate wells. Human IL-8 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human IL-8.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Human IL-8. The concentration of Human IL-8 in the samples is then determined by comparing the OD of the samples to the standard curve.

Cross reactivity: This kit recognizes Human IL-8 in samples.No significant cross-reactivity or interference between Human IL-8 and analogues was observed

• SKU: ELSE-OSEL-H0014-48
• Manufacturer: Elabscience Biotechnology
• Manufacturer SKU: ELA-E-OSEL-H0014-48
• Package Unit: 48 T
• Quantity Unit: PAK
• Application: ELISA