Usage: This ELISA kit applies to the in vitro quantitative determination of Human NGB concentrations in Serum, plasma and other biological fluids.
Tested Sample Types: Serum, plasma and other biological fluids
Assay Time: 3.5h
Sensitivity: 18.75 pg/mL
Detection Range: 31.25-2000 pg/mL
UNIProt ID: Q9NPG2
Test Principle: This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human NGB. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NGB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NGB, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human NGB. You can calculate the concentration of Human NGB in the samples by comparing the RLU value of the samples to the standard curve.
Cross reactivity: This kit recognizes Human NGB in samples.No significant cross-reactivity or interference between Human NGB and analogues was observed