Abbreviation: SOD3
Target Synonym: EC-SOD;Extracellular Superoxide Dismutase [Cu-Zn];
Target Species: Human
Usage: This ELISA kit applies to the in vitro quantitative determination of Human SOD3 concentrations in serum, plasma and other biological fluids.
Tested Sample Types: serum, plasma and other biological fluids
Assay Time: 2.5h
Sensitivity: 3.75 ng/mL
Detection Range: 6.25-400 ng/mL
UNIProt ID: P08294
Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human SOD3. During the reaction, Human SOD3 in the sample or standard competes with a fixed amount of Human SOD3 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human SOD3. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human SOD3 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Cross reactivity: This kit recognizes Human SOD3 in samples.No significant cross-reactivity or interference between Human SOD3 and analogues was observed
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