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Applications: Isolate T cells by depleting other immune cells from a mixed population such as PBMCs.Isolated T cells may be used for downstream applications such as T cell activation, TDCC (T-cell dependent cellular cytotoxicity) assays, genomic analysis, expression assays, protein isolation, and flow cytometry.
Background: T cells are important immune cells that have a variety of functions. T cells can wipe out infected or cancerous cells. They also direct the immune response by helping B lymphocytes to eliminate invading pathogens. CD3 is a common T cell antigen while CD4 and CD8 are antigens present on the surface of T-helper and cytotoxic T cells, respectively. In PBMCs derived from healthy individuals, 45-70% of the cells are T cells.
Description: The Human T Cell Isolation Kit is designed to magnetically separate T cells from a complex immune cell population. This kit is optimized for the negative selection of CD3+ T cells from normal human peripheral blood mononuclear cells (PBMCs). Cells are incubated with a mix of antibodies followed by conjugation to magnetic beads. They are then placed on a magnet for quick and easy separation. When placed on the magnet, non-T cells will be immobilized along the side of the tube while untouched T cells will remain in suspension for downstream use.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed
Supplied As: This kit is provided with enough reagents and materials for isolation of T cells from up to 1 x 108 PBMCs. It is possible to use this kit for multiple isolations from smaller PBMC amounts.
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
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