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Applications: This kit is useful for screening for inhibitors of LAG3 binding to FGL1.
Background: Lymphocyte-activation gene 3 (LAG3, also known as CD223) is a cell surface receptor that negatively regulates the activation and proliferation of T cells. Fibrinogen-like protein 1 (FGL1), a liver-secreted protein, is a functional LAG3 ligand. Blockade of the FGL1-LAG3 interaction is implicated in promoting antitumor immunity.
Description: The LAG3:FGL1[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of LAG3:FGL1. The key to this kit is the high sensitivity of detection of biotin-labeled FGL1 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, LAG3 is attached on a nickel-coated 96-well plate. Next, biotinylated FGL1 is incubated with LAG3 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.
Storage Stability: Up to 6 months from date of receipt, when stored as recommended.
Supplied As: This kit comes in a convenient 96-well format, with biotin-labeled FGL1, purified His-tagged LAG3, streptavidin-labeled HRP, and assay buffer for 100 binding reactions.
Biosafety Level: Not applicable (BSL-1)
References: Wang, J., et al. Immunol. 2019, 156.1: 74-85
Xu, W., et al. Cell. Mol. Immunol. 2018, 15(5): 438
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