Target Synonym: LPO
Result Type: Cell-based (quantitative)
Detection Instrument: Fluorescence Microplate reader
Tested Sample Types: cell
Assay Time: 60 min
Test Principle: Lipid peroxides (LPO) are substances produced by the action of reactive oxygen species (ROS) on unsaturated fatty acids. The LPO produced by the reaction will continue to oxidize the liposomes, thereby accumulating LPO in a chain reaction. Excess LPO decomposes into highly active aldehydes such as Acrolein, Malondialdehyde (MDA), and 4-hydroxy-2-nonenal (4-NHE), causing cell toxicity and cell death.This kit provides a fluorescent probe that can detect LPO production. This fluorescent probe can react with the lipid free radicals in the lipid peroxidation pathway, so as to detect the level of lipid peroxidation in cells. The probe fluoresces red under normal conditions through photoexcitation, and the fluorescence changes from red to green with the process of lipid peroxidation. Through the enhancement of green fluorescence intensity, the production of LPO can be detected with high sensitivity.
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