Abbreviation: β-CTx
Target Synonym: b-CTx;bCTXI;bCTX-I;B-Cr;BCL;Type I Collagen C-Telopeptide-Related Fraction;
Target Species: Monkey
Usage: This ELISA kit applies to the in vitro quantitative determination of Monkey β-CTx concentrations in ..
Tested Sample Types: serum, plasma, other biological fluids
Assay Time: 1.5h
Sensitivity: 75 pg/mL
Detection Range: 125-8000 pg/mL
UNIProt ID: F7BJV7(1207-1214aa)
Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Monkey β-CTx. During the reaction, Monkey β-CTx in the sample or standard competes with a fixed amount of Monkey β-CTx on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Monkey β-CTx. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Monkey β-CTx in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Cross reactivity: This kit recognizes Monkey β-CTx in samples.No significant cross-reactivity or interference between Monkey β-CTx and analogues was observed
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