Monkey F12(Coagulation Factor XII) ELISA Kit

Abbreviation: F12

Target Synonym: F12;HAE3;HAEX;HAF;coagulation factor XII;

Target Species: Monkey

Usage: This ELISA kit applies to the in vitro quantitative determination of Monkey F12 concentrations in Serum, plasma and other biological fluids.

Tested Sample Types: Serum, plasma and other biological fluids

Assay Time: 3.5h

Sensitivity: 0.94 ng/mL

Detection Range: 1.56-100 ng/mL

UNIProt ID: A0A2K5V365

Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey F12. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey F12 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey F12, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Monkey F12. You can calculate the concentration of Monkey F12 in the samples by comparing the OD of the samples to the standard curve.

Cross reactivity: This kit recognizes Monkey F12 in samples. No significant cross-reactivity or interference between Monkey F12 and analogues was observed

• SKU: ELSE-EL-MK0488-48
• Manufacturer: Elabscience Biotechnology
• Manufacturer SKU: ELA-E-EL-MK0488-48
• Green Labware: No
• Package Unit: 48 T
• Quantity Unit: PAK
• Application: ELISA