Abbreviation: Cort
Target Synonym: Corticosterone;CORT;
Target Species: Mouse
Usage: This ELISA kit applies to the in vitro quantitative determination of Mouse Cort concentrations in serum, plasma, saliva, urine.
Tested Sample Types: serum, plasma, saliva, urine
Assay Time: 1.5h
Sensitivity: 4.98 ng/mL
Detection Range: 12.5-800 ng/mL
Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse Cort. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse Cort are added to the micro ELISA plate wells. Mouse Cort in samples (or standards) competes with a fixed amount of Cort on the solid phase supporter for sites on the HRP linked detection antibody specific to Cort. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Mouse Cort in the samples is then determined by comparing the OD of the samples to the standard curve.
Cross reactivity: This kit recognizes Mouse Cort in samples.No significant cross-reactivity or interference between Mouse Cort and analogues was observed
English
Deutsch
