Mouse IgM (Immunoglobulin M) CLIA Kit

Abbreviation: IgM

Target Synonym: IgM

Target Species: Mouse

Usage: This ELISA kit applies to the in vitro quantitative determination of Mouse IgM concentrations in serum, plasma and other biological fluids.

Tested Sample Types: serum, plasma and other biological fluids

Assay Time: 3.5h

Sensitivity: 3.75 ng/mL

Detection Range: 6.25-400 ng/mL

UNIProt ID: P01872

Test Principle: This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IgM. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IgM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IgM, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse IgM. You can calculate the concentration of Mouse IgM in the samples by comparing the RLU value of the samples to the standard curve.

Cross reactivity: This kit recognizes Mouse IgM in samples.No significant cross-reactivity or interference between Mouse IgM and analogues was observed

• SKU: ELSE-CL-M0430-48
• Manufacturer: Elabscience Biotechnology
• Manufacturer SKU: ELA-E-CL-M0430-48
• Green Labware: No
• Package Unit: 48 T
• Quantity Unit: PAK
• Application: Chemiluminescence Immunoassays (CLIA)