Mouse TIMP-1(Tissue Inhibitors of Metalloproteinase 1) ELISA Kit

Abbreviation: TIMP-1

Target Synonym: Calcitonin receptor;Calcitonin receptor precursor;CALCR;CRT;CT-R;CTR;CTR1;

Target Species: Mouse

Usage: This ELISA kit applies to the in vitro quantitative determination of Mouse TIMP-1 concentrations in serum, plasma and other biological fluids.

Tested Sample Types: serum, plasma and other biological fluids

Assay Time: 3.5h

Sensitivity: 46.88 pg/mL

Detection Range: 78.13-5000 pg/mL

UNIProt ID: P12032

Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TIMP-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TIMP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TIMP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse TIMP-1. You can calculate the concentration of Mouse TIMP-1 in the samples by comparing the OD of the samples to the standard curve.

Cross reactivity: This kit recognizes Mouse TIMP-1 in samples.No significant cross-reactivity or interference between Mouse TIMP-1 and analogues was observed

• SKU: ELSE-EL-M3071-48
• Manufacturer: Elabscience Biotechnology
• Manufacturer SKU: ELA-E-EL-M3071-48
• Green Labware: No
• Package Unit: 48 T
• Quantity Unit: PAK
• Application: ELISA