Abbreviation: ACTH
Target Synonym: Corticotropin;POMC;Adrenocorticotropin;
Target Species: Porcine
Usage: This ELISA kit applies to the in vitro quantitative determination of Porcine ACTH concentrations in ..
Tested Sample Types: serum, plasma, other biological fluids
Assay Time: 1.5h
Sensitivity: 9.38 pg/mL
Detection Range: 15.63-1000 pg/mL
UNIProt ID: P01192(136-174aa)
Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Porcine ACTH. During the reaction, Porcine ACTH in the sample or standard competes with a fixed amount of Porcine ACTH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Porcine ACTH. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Porcine ACTH in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Cross reactivity: This kit recognizes Porcine ACTH in samples.No significant cross-reactivity or interference between Porcine ACTH and analogues was observed
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