Usage: This ELISA kit applies to the in vitro quantitative determination of Porcine T concentrations in serum, plasma.
Tested Sample Types: serum, plasma
Assay Time: 1.5h
Sensitivity: 0.28 ng/mL
Detection Range: 0.63-40 ng/mL
Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Porcine T. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Porcine T are added to the micro ELISA plate wells. Porcine T in samples (or standards) competes with a fixed amount of T on the solid phase supporter for sites on the HRP linked detection antibody specific to T. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Porcine T in the samples is then determined by comparing the OD of the samples to the standard curve.
Cross reactivity: This kit recognizes Porcine T in samples.No significant cross-reactivity or interference between Porcine T and analogues was observed