Formula Weight: 0
Shelf life (days): 365
Notes: Quansys Biosciences’ SARS-CoV-2 Human IgG (4-Plex) Qualitative (EUA) Q-Plex multiplex assay is a qualitative enzyme-linked immunosorbent assay that detects IgG antibodies to SARS-CoV-2 in human serum or plasma. SARS-CoV-2 is the novel coronavirus that causes COVID-19. The SARS-CoV-2 virus has several structural proteins including two spike proteins, S1 and S2. When an individual is infected with the SARS-CoV-2 virus, their immune system produces antibodies to these viral proteins. The typical immune response produces detectable antibody levels ~8-10 days following the onset of symptoms. The SARS-CoV-2 Human IgG (4-Plex) Qualitative (EUA) Q-Plex Multiplex ELISA detects IgG antibodies to both the S1 and S2 spike proteins present in a blood sample. If antibodies are detected, the result will be considered positive. If antibodies are not detected, the result will be considered negative. The assay only detects IgG antibodies, not other classes of antibodies such as IgM or IgA. The multiplex assay allows for simultaneous indirect ELISA on the following for printed spots: SARS-CoV-2 Spike Glycoprotein (S1), a recombinant antigen which contains amino acids 1-674 of subunit 1. Spike S1 is expressed in mammalian HEK293 cells with a Sheep Fc-Tag. SARS-CoV-2 Spike Glycoprotein (S2) is a recombinant antigen which contains the Spike subunit 2 protein, amino acids 685-1211. Spike S2 is expressed in mammalian HEK293 cells with a Sheep Fc-Tag. Sheep Fc, is a negative control to ensure no cross-reactivity occurs between human IgGs in the sample and the Fc-Tag on the SARS-Cov-2 Spike proteins. Anti-Human IgG is a positive control to ensure the kit performs and the IFU was followed correctly. Q-Plex™ plates are built by adsorbing multiple distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results. See Item No. 28264 to view pricing and specifications for the Q-View™ Imager LS. Species: Human Assay Type: Indirect Sample Type: Human Serum and Plasma Assay Length: 2 hours Specificity: See Assay Validation Volume Required per Well: 2 µl, minimum Detection Method: Q-View™ Chemiluminescent Imaging System Item No. 28264 Multiplex Format: 96-well solid plate [Quansys Catalog No. 691649HU]
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