Abbreviation: IgM
Target Species: Human
Usage: This ELISA kit applies to the in vitro quantitative determination of Human IgM concentrations in Serum, Plasma, Urine, Saliva.
Tested Sample Types: Serum, Plasma, Urine, Saliva
Assay Time: 2.5h
Sensitivity: 1.02 ng/mL
Detection Range: 7.8-500 ng/mL
Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IgM. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IgM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IgM, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human IgM. You can calculate the concentration of Human IgM in the samples by comparing the OD of the samples to the standard curve.
Cross reactivity: This kit recognizes Human IgM in samples.No significant cross-reactivity or interference between Human IgM and analogues was observed
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