Abbreviation: NGAL
Target Synonym: LCN2;Lipocalin 2;Oncogene 24p3;MSFI;
Target Species: Human
Usage: This ELISA kit applies to the in vitro quantitative determination of Human NGAL concentrations in serum, plasma, urine.
Tested Sample Types: serum, plasma, urine
Assay Time: 2.5h
Sensitivity: 0.06 ng/mL
Detection Range: 0.13-8 ng/mL
UNIProt ID: P80188
Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NGAL. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NGAL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NGAL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human NGAL. You can calculate the concentration of Human NGAL in the samples by comparing the OD of the samples to the standard curve.
Cross reactivity: This kit recognizes Human NGAL in samples.No significant cross-reactivity or interference between Human NGAL and analogues was observed
English
Deutsch
