QuicKey Pro Human E-Cad(E-Cadherin) ELISA Kit

Abbreviation: E-Cad

Target Synonym: CDH1; Arc-1; CD324; CDHE; LCAM; UVO; CAM 120/80; Epithelial Cadherin; Uvomorulin

Target Species: Human

Usage: This ELISA kit applies to the in vitro quantitative determination of Human E-Cad concentrations in serum, plasma and other biological fluids.

Tested Sample Types: serum, plasma and other biological fluids

Assay Time: 1.5h

Sensitivity: 46.88 pg/mL

Detection Range: 78.13-5000 pg/mL

UNIProt ID: P12830

Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human E-Cad. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human E-Cad are added to the micro ELISA plate wells. Human E-Cad in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human E-Cad.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Human E-Cad. The concentration of Human E-Cad in the samples is then determined by comparing the OD of the samples to the standard curve.

Cross reactivity: This kit recognizes Human E-Cad in samples. No significant cross-reactivity or interference between Human E-Cad and analogues was observed.

• SKU: ELSE-OSEL-H0017-96
• Manufacturer: Elabscience Biotechnology
• Manufacturer SKU: ELA-E-OSEL-H0017-96
• Package Unit: 96 T
• Quantity Unit: STK
• Application: ELISA