QuicKey Pro Human ICAM-1/CD54(intercellular adhesion molecule 1) ELISA Kit

Abbreviation: ICAM-1

Target Synonym: CD54

Target Species: Human

Usage: This ELISA kit applies to the in vitro quantitative determination of Human ICAM-1 concentrations in serum, plasma and other biological fluids.

Tested Sample Types: serum, plasma and other biological fluids

Assay Time: 1.5h

Sensitivity: 0.15 ng/mL

Detection Range: 0.25-16 ng/mL

UNIProt ID: P05362

Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ICAM-1. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human ICAM-1 are added to the micro ELISA plate wells. Human ICAM-1 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human ICAM-1.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Human ICAM-1. The concentration of Human ICAM-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Cross reactivity: This kit recognizes Human ICAM-1 in samples. No significant cross-reactivity or interference between Human ICAM-1 and analogues was observed.

• SKU: ELSE-OSEL-H0018-48
• Manufacturer: Elabscience Biotechnology
• Manufacturer SKU: ELA-E-OSEL-H0018-48
• Green Labware: No
• Package Unit: 48 T
• Quantity Unit: PAK
• Application: ELISA