Rat bFGF/FGF2 (Basic Fibroblast Growth Factor) CLIA Kit

Abbreviation: bFGF;FGF2

Target Synonym: FGF-2;B-FGF;BFGF;FGFB;HBGF-2;prostatropin;heparin-binding growth factor 2;

Target Species: Rat

Usage: This ELISA kit applies to the in vitro quantitative determination of Rat bFGF/FGF2 concentrations in serum, plasma and other biological fluids.

Tested Sample Types: serum, plasma and other biological fluids

Assay Time: 3.5h

Sensitivity: 4.69 pg/mL

Detection Range: 7.81-500 pg/mL

UNIProt ID: P13109

Test Principle: This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat bFGF/FGF2. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat bFGF/FGF2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat bFGF/FGF2, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat bFGF/FGF2. You can calculate the concentration of Rat bFGF/FGF2 in the samples by comparing the RLU value of the samples to the standard curve.

Cross reactivity: This kit recognizes Rat bFGF/FGF2 in samples.No significant cross-reactivity or interference between Rat bFGF/FGF2 and analogues was observed

• SKU: ELSE-CL-R0067-48
• Manufacturer: Elabscience Biotechnology
• Manufacturer SKU: ELA-E-CL-R0067-48
• Green Labware: No
• Package Unit: 48 T
• Quantity Unit: PAK
• Application: Chemiluminescence Immunoassays (CLIA)