Abbreviation: CRH
Target Synonym: CRF;Corticoliberin;
Target Species: Rat
Usage: This ELISA kit applies to the in vitro quantitative determination of Rat CRH concentrations in serum, plasma and other biological fluids.
Tested Sample Types: serum, plasma and other biological fluids
Assay Time: 2.5h
Sensitivity: 0.19 ng/mL
Detection Range: 0.31-20 ng/mL
UNIProt ID: P01143
Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat CRH. During the reaction, Rat CRH in the sample or standard competes with a fixed amount of Rat CRH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat CRH. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat CRH in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Cross reactivity: This kit recognizes Rat CRH in samples.No significant cross-reactivity or interference between Rat CRH and analogues was observed
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