Rat IL-12 p40(Interleukin 12 p40) CLIA Kit

Abbreviation: IL-12p40

Target Synonym: IL12B;IL-12B;CLMF2;NKSF2;p40;IL-12/IL-23 p40;

Target Species: Rat

Usage: This ELISA kit applies to the in vitro quantitative determination of Rat IL-12 p40 concentrations in Serum, plasma and other biological fluids.

Tested Sample Types: Serum, plasma and other biological fluids

Assay Time: 3.5h

Sensitivity: 1.88 pg/mL

Detection Range: 3.13-200 pg/mL

UNIProt ID: Q9R278

Test Principle: This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-12 p40. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-12 p40 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-12 p40, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat IL-12 p40. You can calculate the concentration of Rat IL-12 p40 in the samples by comparing the RLU value of the samples to the standard curve.

Cross reactivity: This kit recognizes Rat IL-12 p40 in samples.No significant cross-reactivity or interference between Rat IL-12 p40 and analogues was observed

• SKU: ELSE-CL-R0051-48
• Manufacturer: Elabscience Biotechnology
• Manufacturer SKU: ELA-E-CL-R0051-48
• Green Labware: No
• Package Unit: 48 T
• Quantity Unit: PAK
• Application: Chemiluminescence Immunoassays (CLIA)