Rat PACAP(Pituitary Adenylate Cyclase Activating Polypeptide) ELISA Kit

Abbreviation: PACAP

Target Synonym: ADCYAP1;PACAP;

Target Species: Rat

Usage: This ELISA kit applies to the in vitro quantitative determination of Rat PACAP concentrations in serum, plasma and other biological fluids.

Tested Sample Types: serum, plasma and other biological fluids

Assay Time: 3.5h

Sensitivity: 4.69 pg/mL

Detection Range: 7.81-500 pg/mL

UNIProt ID: P13589

Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat PACAP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PACAP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PACAP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat PACAP. You can calculate the concentration of Rat PACAP in the samples by comparing the OD of the samples to the standard curve.

Cross reactivity: This kit recognizes Rat PACAP in samples. No significant cross-reactivity or interference between Rat PACAP and analogues was observed

• SKU: ELSE-EL-R2387-48
• Manufacturer: Elabscience Biotechnology
• Manufacturer SKU: ELA-E-EL-R2387-48
• Package Unit: 48 T
• Quantity Unit: PAK
• Application: ELISA