Abbreviation: E2
Target Synonym: Estradiol;E2;
Target Species: Rat, Porcine
Usage: This ELISA kit applies to the in vitro quantitative determination of Rat, Porcine E2 concentrations in serum, plasma.
Tested Sample Types: serum, plasma
Assay Time: 2.5h
Sensitivity: 1 pg/mL
Detection Range: 1.56-100 pg/mL
Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat, Porcine E2. During the reaction, Rat, Porcine E2 in the sample or standard competes with a fixed amount of Rat, Porcine E2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat, Porcine E2. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat, Porcine E2 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Cross reactivity: This kit recognizes Rat, Porcine E2 in samples.No significant cross-reactivity or interference between Rat, Porcine E2 and analogues was observed
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