Abbreviation: VIP
Target Synonym: PHM27
Target Species: Rat
Usage: This ELISA kit applies to the in vitro quantitative determination of Rat VIP concentrations in Serum, plasma and other biological fluids.
Tested Sample Types: Serum, plasma and other biological fluids
Assay Time: 2.5h
Sensitivity: 4.69 pg/mL
Detection Range: 7.81-500 pg/mL
UNIProt ID: P01283(125-152)
Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat VIP. During the reaction, Rat VIP in the sample or standard competes with a fixed amount of Rat VIP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat VIP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat VIP in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Cross reactivity: This kit recognizes Rat VIP in samples.No significant cross-reactivity or interference between Rat VIP and analogues was observed
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