Abbreviation: ST;5-HT
Target Synonym: 5HT;Enteramine;Thrombocytin;Thrombotonin;3-(β-Aminoethyl)-5-hydroxyindole;
Target Species: Universal
Usage: This ELISA kit applies to the in vitro quantitative determination of ST/5-HT concentrations in serum, plasma and other biological fluids.
Tested Sample Types: serum, plasma and other biological fluids
Assay Time: 2.5h
Sensitivity: 9.38 ng/mL
Detection Range: 15.63-1000 ng/mL
Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal ST/5-HT. During the reaction, Universal ST/5-HT in the sample or standard competes with a fixed amount of Universal ST/5-HT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal ST/5-HT. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal ST/5-HT in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Cross reactivity: This kit recognizes Universal ST/5-HT in samples.No significant cross-reactivity or interference between Universal ST/5-HT and analogues was observed
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