Human WGA Non-Methylated DNA (5 μg / 20 μl)

This control set is generated from HCT116 DKO cell genomic DNA using whole-genome amplification (WGA) technology. This standard is ideal for studying loci with ultra-low methylation levels.

Description: The Human WGA Methylated & Non-methylated DNA Set consists of two control DNAs (non-methylated and methylated) along with a set of specifically designed primers that can be used in conjunction with the EZ DNA Methylation, EZ DNA Methylation-Gold, EZ DNA Methylation-Direct, and EZ DNA Methylation-Lightning kits from Zymo Research to assess the efficiency of bisulfite-mediated conversion of DNA. The Human WGA Methylated & Non-methylated DNA Set is generated using phi29 DNA polymerase based whole genome amplification techniques from HCT116 DKO cell line derived genomic DNA (Human HCT116 DKO Non-methylated DNA). The Human WGA Methylated DNA is Human WGA Non-methylated DNA that has been enzymatically methylated at all double-stranded CG dinucleotides using M.SssI methyltransferase and can be used as a positive control for DNA methylation analysis.

• Purified, non-methylated and methylated human DNA for use as negative and positive control in methylation detection applications.
• Standards can be assayed in parallel with samples to monitor bisulfite conversion efficiency.
• Ideal controls for bisulfite sequencing PCR (BSP) and methylation specific PCR (MSP). Primers included with the full set are designed to amplify non-methylated, methylated, and mixed methylated copies of the Ras association (RalGDS/AF-6) domain family member 1 (RASSF1) gene following bisulfite conversion.

• SKU: ZYMD5013-1
• Manufacturer: Zymo Research
• Manufacturer SKU: D5013-1
• Green Labware: No
• Package Unit: 1 PCE
• Quantity Unit: STK