Methylated & Non-Methylated pUC19 DNA Set

Control for bisulfite conversion and DNA methylation quantitation

Description: The Methylated & Non-methylated pUC19 DNA Set consists of control DNAs and a set of specifically designed primers. The set is ideal as a spike-in control to assess bisulfite conversion efficiency within the same reaction as the sample, or to produce known mixtures of methylated and non-methylated DNA for assay calibration. The non-methylated pUC19 DNA is pUC19 isolated from a methylation-negative strain of bacteria (Dam-, Dcm-), and the methylated pUC19 DNA is pUC19 enzymatically methylated at all cytosines in the dinucleotide sequence 5'...CpG...3' by CpG Methylase.

• Includes pUC19MN primers in 20 µl

• SKU: ZYMD5017
• Manufacturer: Zymo Research
• Manufacturer SKU: D5017
• Green Labware: No
• Package Unit: 1 PCE
• Quantity Unit: STK