BRD4 bromodomain 1 TR-FRET Assay Kit

BRD4 bromodomain 1 TR-FRET Assay Kit
SKU
CAY600520-9600
Packaging Unit
9600 wells
Manufacturer
Cayman Chemical

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Shelf life (days): 365

Notes: Cayman’s BRD4 bromodomain 1 TR-FRET Assay Kit is a homogeneous, time-resolved Förster resonance energy transfer (TR-FRET) assay method amenable to rapid characterization of inhibitors of the bromodomain-acetylated peptide interaction in a high-throughput format. The donor fluorophore in this assay consists of BRD4 bromodomain 1 (human amino acids 49-170) directly labeled with a europium (Eu3+) chelate. A biotinylated peptide containing acetylated lysines (BRD-Eu3+) serves as the ligand for BRD4 bromodomain 1. Allophycocyanin (APC)-labeled avidin binds with high affinity to the peptide substrate via the biotin moiety and serves as the acceptor fluorophore in the assay. Inhibition of the BRD-peptide interaction displaces BRD-Eu3+ from the APC-avidin conjugate resulting in a loss of TR-FRET signal. The BRD4 bromodomain 1 TR-FRET Assay Kit is robust (Z' = >0.85) and suitable for high-throughput screening in the provided 384-well plate or can be scaled to higher density plate formats (e.g., 1,536-well plate) if desired. The assay is stable at room temperature for at least four hours and in the presence of less than 2% DMSO.
More Information
SKU CAY600520-9600
Manufacturer Cayman Chemical
Manufacturer SKU 600520-9600
Package Unit 9600 wells
Quantity Unit PAK
Application TR-FRET Assay, Fluorescence, High-Throughput Screening
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