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Application: Screen and characterize modulators of the estrogen receptor signaling pathway.Generate estrogen response element luciferase reporter cell pools or stable cell lines following puromycin selection.
Background: ER (estrogen receptor) exists as two nuclear ER isoforms (ERα and ERβ) and as a membrane associated ER (GPER1), and responds to estrogen. Estrogen is a hormone produced mainly in the ovaries, but also in the adipose tissue, and plays a role in reproduction, bone density, inflammation, and others. Estrogen can penetrate the cell membrane and bind to ERα, which dimerizes and translocates to the nucleus, where it associates with transcriptional co-activators and binds to ERE (estrogen response elements). In addition to this genomic role, ER also plays non genomic roles by directly activating cellular signaling pathways. About 60% of breast cancer (BC) cases are ERα-positive and are classified as luminal BC. Patients with luminal BC have several therapeutic strategies available to them: hormone therapy, SERM (selective estrogen receptor modulators), AI (aromatase inhibitors), SERD (selective estrogen receptor degraders) and LHRH (luteinizing hormone-releasing hormone). However, about half of the metastatic therapy-resistant BC result from ERα mutations, with at least 62 being known. New therapeutic avenues are thus needed for the successful treatment of this BC patient population.
Description: Estrogen Response Element (ERE) Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce most mammalian cells, including primary and non-dividing cells. These viruses contain a firefly luciferase reporter driven by multiple copies of estrogen response element (ERE) located upstream of the minimal TATA promoter. The lentiviruses also contain a puromycin selection marker . After transduction, the cellular estrogen receptor signaling pathway can be monitored by measuring luciferase activity.
Formulation: The lentivirus particles were produced from HEK293T cells. They are supplied in cell culture medium containing 90% DMEM + 10% FBS. Virus particles can be packaged in custom formulations by special request, for an additional fee.
Storage Stability: Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles. Titers can drop significantly with each freeze/thaw cycle.
Supplied As: Two vials (500 µl x 2) of lentivirus at a titer >107 TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.
Warnings: Avoid freeze/thaw cycle
Biosafety Level: BSL-2
References: Juliette L., et al., 1999 Toxicological Sciences 48: 55-66.
Yu S., et al., 2017 Biochemical and Biophysical Research Communications 486(3): 752-758.
Clusan L., et al., 2023 Int J Mol Sci 24(7): 6834.
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