Products from BPS Bioscience require a minimum order value above 400€
Applications: • Expression of human HLA-E in cells of interest.• Generate cell pools or stable cell lines expressing HLA-E following puromycin selection.
Background: HLA-E, or MHC (major histocompatibility complex) class I antigen E, is considered a non-classical MHC class I with low expression and fewer polymorphisms than the remaining HLA. HLA-E is composed of a heavy chain and β-2 microglobulin (B2M). It binds to specific peptides derived from the classical MHC class I (HLA-A, B, C and G), after these have been processed in the endoplasmic reticulum and the proteosome. The complex of HLA-E with the peptide is recognized by NK cells via the inhibitory receptor CD94/NKG2A/B. Binding to CD94/NKG2C however results in NK cell activation. Expression of HLA-E combined with knockout of HLA-A, B and C, in pluripotent stem cell (PSC) and their differentiated cell types, resulted in these cells escaping attack by CD8+ T cells and NK cytotoxicity. This strategy brings us closer to an almost universal cell donor reality, reducing the risk of immune rejection during cell transplants and alleviating the enormous investment of creating a PSC bank that has representation of all the haplotypes.
Description: HLA-E Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles ready to transduce nearly all types of mammalian cells, including primary and non-dividing cells. These viruses result in expression of human HLA-E (NM_005516.6) driven by an EF1a promoter, and a puromycin selection marker.
Formulation: The lentivirus particles were produced in HEK293T cells in medium containing 90% DMEM + 10% FBS. Virus particles can be packaged in custom formulations and produced at higher titers by special request, for an additional fee.
Storage Stability: Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle.
Supplied As: Two vials (500 µl x 2) of lentivirus at a titer ≥107 TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.
Warnings: Avoid freeze/thaw cycles
Biosafety Level: BSL-2
References: Gornalusse G., et al., 2017 Nature Biotechnology 35:765-772
English
Deutsch
