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Applications: Study CD4-MHC class II interactions.Generation of cell pools or stable cell lines expressing CD4 following hygromycin selection.
Background: CD4 is a cell surface glycoprotein found on partially defined functional T-cell subsets, including helper T cells and T-regulatory cells, peripheral monocytes and other APCs (antigen-presenting cells). The ectodomain of CD4 binds to membrane-proximal domains of MHC (major histocompatibility complex) class II molecules, while its cytoplasmic domains interact with the protein tyrosine kinase p56lck (lck) through a shared cysteine-containing motif. CD4+ T cells, or helper T cells, are one type of lymphocyte that helps coordinate the immune response against infection and disease by activating cells of the innate immune system, B-lymphocytes and cytotoxic T cells. CD4+ T cells are activated by interaction between the TCR (T- cell receptor) and its cognate peptide presented on MHC II molecules, and CD4 is a critical component of the T cell receptor complex that recognizes antigenic peptides presented by MHC II molecules, increasing its stability. CD4 is a typically used T cell marker that allows to characterize the populations of T cells present.
Description: The Human CD4 Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain CD4 (NM_000616.5) driven by an EF1a promoter, and a hygromycin selection marker.
Formulation: The lentiviruses were produced from HEK293T cells. Supplied in medium containing 90% DMEM + 10% FBS. Virus particles can be packaged in custom formulations and produced at higher titers by special request, for an additional fee.
Storage Stability: Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C for up to 12 months from date of receipt. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle.
Supplied As: Two vials (500 µl x 2) of lentiviruses at a titer ≥107 TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.
Uniprot: P01730
Warnings: Avoid freeze/thaw cycles
Biosafety Level: BSL-2
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