Products from BPS Bioscience require a minimum order value above 400€Applications: Generate stable cell line expressing human LYPD1 with puromycin selection
Background: LY6/PLAUR domain containing 1 (LYPD1, also known as Lynx2) is a GPI-anchored membrane protein involved in acetylcholine receptor signaling pathway and cholinergic synaptic transmission. High levels of expression have been observed in areas of the brain associated with depression. In addition, mice in which the LYPD1 gene was deleted show increased anxiety-related behaviors.
Description: The LYPD1 Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce nearly all types of mammalian cells, including primary and non-dividing cells. The particles contain a human LYPD1 (NM_ 001321234.2) driven by a EF1A promoter and a puromycin selection marker (Figure 1).
Figure 1: Schematic of the lenti-vector used to generate the LYPD1 Lentivirus
Formulation: The lentivirus particles were produced from HEK293T cells. They are supplied in cell culture medium containing 90% DMEM + 10% FBS.
Storage Stability: Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C.
Supplied As: Two vials (500 µl x 2) of lentivirus at a titer ≥10
7 TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.
Uniprot: Q8N2G4
Warnings: Avoid freeze/thaw cycles
Biosafety Level: BSL-2
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