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Application: Use as a positive control for PRAME TCR evaluation and optimize experimental conditions.Generate PRAME TCR expressing cell pools or stable cell lines, following puromycin selection.
Background: PRAME (Preferentially Expressed Antigen in Melanoma) is a protein with a profile of expression in normal tissues highly restricted to testis, ovarium, and endometrium. However, it is found at high levels in several cancer types, such as melanoma, breast, and lung cancer. It is also found in cells of patients with AML (acute myeloid leukemia) and Hodgkin's lymphoma. Overexpression seems to block retinoic acid-mediated cell proliferation, differentiation, and apoptosis, contributing to tumorigenesis. Its expression pattern makes it an attractive target for immunotherapy. It is a membrane-bound protein, and it is thus a good target for TCR (T cell receptor)-T cells and anti-PRAME vaccines. Several clinical trials are ongoing and have demonstrated the clinical potential of targeting PRAME in melanoma, lung cancer and other solid tumors. Further studies into the functions of this protein will bring new clinical advances in cancer therapy.
Description: PRAME-Specific TCR Lentivirus are replication incompetent, HIV-based, VSV-G-pseudotyped lentiviral particles ready to transduce nearly all types of mammalian cells, including primary and non-dividing cells. These viruses transduce cells with a TCR (T cell receptor) that specifically recognizes the human antigen PRAME (Preferentially Expressed Antigen in Melanoma) peptide 425-433 (SLLQHLIGL), and in which the TCR α chain and β chain are linked by P2A. The lentiviruses also transduce a puromycin selection marker.
Formulation: The lentiviruses were produced in HEK293T cells in medium containing 90% DMEM + 10% FBS. Virus particles can be packaged in custom formulations by special request, for an additional fee.
Storage Stability: Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the virus at -80°C for up to 12 months from date of receipt. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle.
Supplied As: ≥2 x 107 TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.
Uniprot: P78395
Warnings: Avoid freeze/thaw cycles
Biosafety Level: BSL-2
References: Xu Y., et al., 2020 Cell Prolif. 53(3):e12770.
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