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Application: Knockout of TCR α/β in cells of interest.
Background: The TCR (T Cell Receptor) is found on the surface of T cells and is responsible for recognizing antigens bound to MHC (Major Histocompatibility Complex) molecules. Stimulation of the TCR results in activation of downstream NFAT (Nuclear factor of activated T-cells) signaling. NFAT is a family of transcription factors that has an important function in immune responses, for example by inducing the expression of various cytokines (such as interleukin-2 to 4, and TNF-alpha (tumor necrosis factor alpha)) in T cells. The TCR consists of a heterodimer of two different protein chains, of which the alpha (α) and beta (β) chains are the predominant chains. Knocking out the TCR molecule in T cells ensures the specificity of engineered TCR or CAR-T cells that target tumor-associated antigens and is a critical step in the development of adoptive cell therapies as the endogenous TCR could interfere by recognizing other antigens not intended for targeting. TCR-KO T cells rely exclusively on the engineered receptor for antigen recognition, thereby improving the specificity and potency of the therapy.
Description: The TCR Knockout Electroporation Kit is suitable for Jurkat cell line and primary T cell engineering using electroporation. The kit contains both the Cas9 enzyme (Streptococcus pyogenes) and the gRNAs targeting TRAC (T-Cell Receptor Alpha Constant) and TRBC (targets both T Cell Receptor Beta Constant 1 and T Cell Receptor Beta Constant 2). This kit is sufficient to engineer up to 5 million primary T cells.
Mycoplasma Testing: Sterile filtered (0.2 µm) and Mycoplasma-free. All components are low endotoxin. FBS is ISIA-certified and sourced from the United States of America.
Storage Stability: Upon receipt, the medium should be stored in the dark at 4°C, the product is stable for six months after first thaw. Avoid repeated freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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