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Applications: Positive control in the transduction of macrophages.Optimization of transduction assays and tracking of transgene expression over time
Background: Adeno-Associated Virus-DJ (AAV-DJ) is a synthetic serotype made from eight different wild-type AAV serotypes (AAV2, 4, 5, 8, 9, avian, bovine, and goat AAV) using DNA shuffling. These modifications allow the AAV-DJ serotype to exhibit improved transduction efficiency in vitro and in vivo and infect a broader range of cell types compared to the wild-type serotypes.CD68 is a protein expressed in macrophages and macrophage-related cells. The CD68 gene promoter directs macrophage-specific expression. Although the promoter lacks a classical TATA box, it contains other protein binding sites consistent with preferential monocyte/macrophage gene expression. CD68 promoter has been used to achieve constitutive expression of IL-10 in macrophages.
Description: These AAV-DJ particles constitutively express the firefly (Photinus pyralis) luciferase under the control of a CD68 (cluster of differentiation 68) promoter.
Formulation: AAV was produced in HEK293-AAV cells and is supplied in PBS-MK (PBS Magnesium-Potassium) buffer containing 0.01% Pluronic F68. Virus particles can be packaged in custom formulations by special request, for an additional fee.
Purification: The purity of the AAV particles was confirmed to be greater than 90% by staining with One-Step Lumitein™ UV Protein Gel Stain (Biotium, 21005-1L). The purity varies with each lot; the exact value will be provided with each shipment.
Storage Stability: AAV is shipped with dry ice. For long-term storage, it is recommended to store AAV at -80°C. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle.
Supplied As: Two vials (50 µl x 2) of AAV at a titer ?1 x 1012 vector genomes/ml. The titer is determined by qPCR and will vary with each lot; the exact value will be provided with each shipment.
Warnings: Avoid freeze/thaw cycles
Biosafety Level: BSL-1
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