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Applications: Use as a negative control for gene-specific shRNA transduction via AAV vectors.
Background: RNA interference (RNAi) is a highly specific tool for protein knockdown in which a small non-coding RNA binds to the target protein's mRNA to promote its degradation and reduce protein translation. AAV vector-based expression of RNAi drives prolonged reduction in the level of mRNA targets in live animals. Short-hairpin RNA is one of the most widely used RNA interference methods to inhibit protein translation.Adeno-Associated Virus-DJ (AAV-DJ) is a synthetic serotype made from eight different wild-type AAV serotypes (AAV2, 4, 5, 8, 9, avian, bovine, and goat AAV) using DNA shuffling. These modifications allow the AAV-DJ serotype to exhibit improved transduction efficiency in vitro and in vivo and infect a broader range of cell types compared to wild-type serotypes.
Description: This AAV-DJ Scrambled shRNA (short-hairpin RNA) Control co-expresses a 29-mer scrambled shRNA sequence under the control of a U6 promoter, and Turbo GFP under the control of a CMV promoter to assess the efficacy of transduction.
Formulation: AAV was produced in HEK293-AAV cells and is supplied in PBS-MK (PBS Magnesium-Potassium) buffer containing 0.01% Pluronic F68. Virus particles can be packaged in custom formulations by special request, for an additional fee.
Purity: The purity of the AAV particles was confirmed to be greater than 90% by staining with One-Step Lumitein™ UV Protein Gel Stain (Biotium, 21005-1L). Purity varies with each lot; the exact value will be provided with each shipment.
Storage Stability: AAV is shipped with dry ice. For long-term storage, it is recommended to store AAV at -80°C. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle.
Supplied As: Two vials (50 µl x 2) of AAV at a titer ≥1 x 1012 vector genomes/ml. The titer is determined by qPCR and varies with each lot; the exact value will be provided with each shipment.
Warnings: Avoid freeze/thaw cycles
Biosafety Level: BSL-1
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